Transcriptional Mechanics

We investigate the basic mechanisms of transcription using various bacterial systems. We are particularly interested in the elaborate mechanics of the elongation phase of the transcription cycle.

Using various biochemical and protein chemical tools developed in our lab over the years, we examine how RNA polymerase moves along its DNA template, how it responds to regulatory factors and signals encoded in RNA and DNA, and how it terminates transcription.

We also study the interplay between transcription and other major cellular processes, such as translation, replication and repair of DNA.

RNA polymerase backtracking. underlies key regulatory mechanism of transcription elongation, such as pausing and termination, and plays critical roles in gene regulation and genome instability